منابع مشابه
Superoxide Flashes in Single Mitochondria
In quiescent cells, mitochondria are the primary source of reactive oxygen species (ROS), which are generated by leakiness of the electron transport chain (ETC). High levels of ROS can trigger cell death, whereas lower levels drive diverse and important cellular functions. We show here by employing a newly developed mitochondrial matrix-targeted superoxide indicator, that individual mitochondri...
متن کاملMitochondrial Flashes: Dump Superoxide and Dance with Protons Now
Transient changes in the physiology of individual mitochondria have recently drawn much interest. The use of a circular permuted yellow fluorescent protein (cpYFP) to monitor mitochondrial flashes and their interpretation as superoxide bursts has added confusion, however. Reviewing mitochondrial flashes in this Forum, Wang et al. again deem cpYFP to be a specific and reversible superoxide indic...
متن کاملMitochondrial superoxide flashes: From discovery to new controversies
Introduction Reactive oxygen species (ROS) and reactive nitrogen species (RNS) generated during normal physiological processes are highly reactive with cellular lipids, DNA, and proteins. Superoxide and nitric oxide are the primary ROS and RNS, respectively, produced in cells, and both species react with other molecules and each other to form a diverse array of additional ROS and RNS (e.g., hyd...
متن کاملProinflammatory Cytokines Stimulate Mitochondrial Superoxide Flashes in Articular Chondrocytes In Vitro and In Situ
OBJECTIVE Mitochondria play important roles in many types of cells. However, little is known about mitochondrial function in chondrocytes. This study was undertaken to explore possible role of mitochondrial oxidative stress in inflammatory response in articular chondrocytes. METHODS Chondrocytes and cartilage explants were isolated from wild type or transgenic mice expressing the mitochondria...
متن کاملSuperoxide Flashes in Mouse Skeletal Muscle Are Produced by Discrete Arrays of Active Mitochondria Operating Coherently
Reactive Oxygen Species (ROS) constitute important intracellular signaling molecules. Mitochondria are admitted sources of ROS, especially of superoxide anions through the electron transport chain. Here the mitochondria-targeted ratiometric pericam (RPmt) was used as a superoxide biosensor, by appropriate choice of the excitation wavelength. RPmt was transfected in vivo into mouse muscles. Conf...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2011
ISSN: 0021-9258
DOI: 10.1074/jbc.m111.241794